PDPK1 antibody (AA 287-336)
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- Target See all PDPK1 Antibodies
- PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
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Binding Specificity
- AA 287-336
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Reactivity
- Human, Mouse, Rat, Monkey, Cow, Dog, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PDPK1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Human PDK1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Bovine, Horse (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa287-336 of human PDK1 (Q15118, NP_002601). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Galago, Mouse, Rat, Bovine, Horse, Opossum, Guinea pig (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product PDPK1 Primary Antibody
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- Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
- Alternative Name
- PDK1 (PDPK1 Products)
- Synonyms
- pdpk1 antibody, MGC82080 antibody, PDK1 antibody, PDPK2 antibody, PRO0461 antibody, Pdk1 antibody, zgc:153787 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antibody, ATPDK1 antibody, AtPDK1 antibody, T32M21.110 antibody, T32M21_110 antibody, zgc:77318 antibody, 3-phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1 L homeolog antibody, 3-phosphoinositide dependent protein kinase-1 antibody, 3-phosphoinositide dependent protein kinase 1a antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1b antibody, PDPK1 antibody, pdpk1.L antibody, pdpk1 antibody, Pdpk1 antibody, pdpk1a antibody, LOC100380750 antibody, pdk-1 antibody, PDK1 antibody, pdpk1b antibody
- Background
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Name/Gene ID: PDK1
Subfamily: PDK/BCKDK
Family: Protein Kinase
Synonyms: PDK1, PDHK, PDH kinase 1, PDHK1 - Gene ID
- 5163
- NCBI Accession
- NP_002601
- UniProt
- Q15118
- Pathways
- PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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