FGF1 antibody (Biotin)
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- Target See all FGF1 Antibodies
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FGF1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human FGF-acidic
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human FGF-acidic.
Type of Immunogen: Cell extract - Top Product
- Discover our top product FGF1 Primary Antibody
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- Application Notes
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hFGF-acidic by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. To detect hFGF-acidic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FGF-acidic (LS-B8222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
- Alternative Name
- FGF1 (FGF1 Products)
- Synonyms
- AFGF antibody, ECGF antibody, ECGF-beta antibody, ECGFA antibody, ECGFB antibody, FGF-1 antibody, FGF-alpha antibody, FGFA antibody, GLIO703 antibody, HBGF-1 antibody, HBGF1 antibody, Fgf1 antibody, FGF1 antibody, aFGF antibody, ecgf antibody, fgfa antibody, ecgfa antibody, ecgfb antibody, fgf-1 antibody, hbgf1 antibody, glio703 antibody, ecgf-beta antibody, fgf-alpha antibody, Dffrx antibody, Fam antibody, Fgf-1 antibody, Fgfa antibody, zgc:136885 antibody, LOC100221393 antibody, fgf1 antibody, zgc:73249 antibody, fibroblast growth factor 1 antibody, fibroblast growth factor 1 L homeolog antibody, fibroblast growth factor 1b antibody, fibroblast growth factor 1a antibody, FGF1 antibody, fgf1.L antibody, fgf1 antibody, Fgf1 antibody, fgf1b antibody, LOC100221393 antibody, fgf1a antibody
- Background
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Name/Gene ID: FGF1
Family: HBGF
Synonyms: FGF1, AFGF, Endothelial cell growth factor, Fibroblast growth factor 1, FGFA, ECGFB, HBGF-1, GLIO703, ECGF, ECGF-beta, ECGFA, FGF-1, FGF-alpha, HBGF1 - Gene ID
- 2246
- UniProt
- P05230
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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