BDNF antibody
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- Target See all BDNF Antibodies
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BDNF antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Human BDNF
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human BDNF.
Type of Immunogen: Cell extract - Top Product
- Discover our top product BDNF Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.54 - 0.81 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with sterile water.
- Concentration
- Lot specific
- Buffer
- Lyophilized, PBS, pH 7.2. Reconstitute with sterile water.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Alternative Name
- BDNF (BDNF Products)
- Synonyms
- ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody
- Background
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Name/Gene ID: BDNF
Family: NGF Beta
Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin - Gene ID
- 627
- UniProt
- P23560
- Pathways
- RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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