VWF antibody
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- Target See all VWF Antibodies
- VWF (Von Willebrand Factor (VWF))
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This VWF antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectrophoresis (IEP), Immunodiffusion (ID)
- Specificity
- Recognizes human Von Willebrand Factor. Identity has been confirmed by double diffusion (Ouchterlony) vs. human vWF and a known anti-human vWF. Gives single arc when tested by IEP against human plasma.
- Purification
- Delipidated
- Immunogen
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Human von Willebrand Factor Antigen (vWF) prepared from citrated human plasma.
Type of Immunogen: Purified protein - Isotype
- IgG
- Top Product
- Discover our top product VWF Primary Antibody
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- Application Notes
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Approved: ID, IE, IHC, IHC-P
Usage: Suitable for use in Immunohistochemistry, Double Diffusion, Counter Current Immunoelectrophoresis (CCIEP) and in a variety of gel techniques. CCIEP: 40 μL antiserum vs. 10 μL plasma, wells 6mm apart. 100v for 30 minutes. The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or Tris-barbital, pH >8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finally staining with a protein stain such as Coomassie blue. Double Diffusion: 20 μL antiserum vs. 5 μL vWF, 1 mg/mL. Immunohistochemistry: Specifically binds to endothelial cells on formalin-fixed, paraffin embedded, sections of human tonsil and pancreas. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- GBS, pH 7.4, 0.1 % sodium azide, 0.1 % EACA, 0.01 % benzamine, 1 mM EDTA.
- Preservative
- Sodium azide, EACA
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- VWF (Von Willebrand Factor (VWF))
- Alternative Name
- VWF / Von Willebrand Factor (VWF Products)
- Synonyms
- VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody
- Background
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Name/Gene ID: VWF
Synonyms: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor - Gene ID
- 7450
- UniProt
- P04275
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