BAD antibody (pSer134)

Catalog No. ABIN482136

This anti-BAD antibody is a Rabbit Polyclonal antibody detecting BAD in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for BAD antibody (pSer134) (ABIN482136)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BAD Antibody

Binding Specificity: AA 100-149, pSer134

Specificity:

BAD (Phospho-Ser134) Antibody detects endogenous levels of BAD only when phosphorylated at Ser134.

PhosphorylationH:S134 M:S170 R:S171

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser134.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Comment:

Unigene-Number: Hs.370254 (NCBI Gene Symbol: BAD)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background:

Synonyms: BAD, BBC6, Bcl- XL/Bcl-2 associated death promoter, Bcl-2 binding component 6, Bcl-2-like 8 protein, Bcl-xL/Bcl-2 associated death promoter, Bcl2-antagonist of cell death, BCL2L8

NCBI Gene Symbol: BAD

Molecular Weight: 22 kDa

Gene ID: 572

OMIM: 603167

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events