AKT1 antibody

Catalog No. ABIN488530

This Rabbit Polyclonal antibody specifically detects AKT1 in WB. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for AKT1 antibody (ABIN488530)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKT1 Antibody

Purpose: Anti-AKT1 | Potassium channel AKT1

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Arabidopsis thaliana

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998

Application Details

Application Notes: 1 : 50 with 125I (WB)

Comment:

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background:

Background: AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.

Additional information: For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1

Molecular Weight: 96.9 kDa

Gene ID: 817206

UniProt: Q38998

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect