DGCR8 antibody (AA 446-460)

Catalog No. ABIN490424

Product Details anti-DGCR8 Antibody

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Binding Specificity: AA 446-460

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Monkey, Xenopus laevis, Bat, Chicken, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: ELISA

Specificity: Human DGCR8.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Chicken, Xenopus (100%) Stickleback, Pufferfish, Zebrafish (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KRFDFEQVTVKKFRT from an internal region of human DGCR8 (NP_073557.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Chicken, Xenopus (100%), Stickleback, Pufferfish, Zebrafish (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx. 75  kDa band in Human Brain (amygdala) lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 86.0  kDa according to NP_073557.3. The 75  kDa band was successfully blocked by incubation with the immunizing peptide.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: DGCR8 (DGCR8 Products)

Synonyms: MGC78846 antibody, DGCR8 antibody, gy1 antibody, dgcrk6 antibody, C22orf12 antibody, DGCRK6 antibody, Gy1 antibody, pasha antibody, D16H22S1742E antibody, D16H22S788E antibody, D16Wis2 antibody, N41 antibody, Vo59c07 antibody, si:ch211-106a19.4 antibody, wu:fc23f08 antibody, wu:fc38f06 antibody, DGCR8 microprocessor complex subunit L homeolog antibody, DiGeorge syndrome critical region gene 8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8, microprocessor complex subunit antibody, microRNA 3618 antibody, dgcr8.L antibody, DGCR8 antibody, dgcr8 antibody, Dgcr8 antibody, MIR3618 antibody

Background: Name/Gene ID: DGCR8

Synonyms: DGCR8, DGCRK6, Gy1, C22orf12, Pasha

Gene ID: 54487

NCBI Accession: NP_073557

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways