IL-18 antibody (AA 123-135)

Catalog No. ABIN490550

Product Details anti-IL-18 Antibody

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Binding Specificity: AA 123-135

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human IL18 / IL-18. This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%) Gorilla (92%) Marmoset (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NPPDNIKDTKSDI from an internal region of human IL18 / IL-18 (NP_001553.1, NP_001230140.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla (92%), Marmoset (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 23  kDa band observed in Human Placenta lysates and in lysates of cell line HeLa (calculated MW of 22.3  kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18 (IL18 Products)

Synonyms: Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody

Background: Name/Gene ID: IL18
Family: IL-1

Synonyms: IL18, IGIF, IL-1g, Interleukin-18, IFN-gamma-inducing factor, IL-1 gamma, IL-18, Iboctadekin, IL1F4, Interleukin-1 gamma

Gene ID: 3606

NCBI Accession: NP_001553, NP_001230140

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome