Mesothelin antibody (AA 302-314)

Catalog No. ABIN490621

Product Details anti-Mesothelin Antibody

Target: Mesothelin (MSLN)

Binding Specificity: AA 302-314

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Human MSLN / Mesothelin. This antibody is expected to recognize isoform 1 and 2 (NP_005814.2, NP_037536.2).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CPSGKKAREIDES from an internal region of human MSLN / Mesothelin (NP_005814.2, NP_037536.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70  kDa band observed in Human Placenta lysates (calculated MW of 68.1  kDa according to NP_005814.2 and of 69.0  kDa according to NP_037536.2). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 150  kDa. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN / Mesothelin (MSLN Products)

Synonyms: MPF antibody, SMRP antibody, MSLN antibody, mesothelin antibody, MSLN antibody, Msln antibody, LOC611363 antibody, LOC100524016 antibody

Background: Name/Gene ID: MSLN

Synonyms: MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1

Gene ID: 10232

NCBI Accession: NP_005814, NP_037536

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process