ATG4D antibody

Catalog No. ABIN4906368

Product Details anti-ATG4D Antibody

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.

Clone: 2C1

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D (ATG4D Products)

Synonyms: APG4-D antibody, APG4D antibody, AUTL4 antibody, 9830134P12Rik antibody, Apg4d antibody, Atg4dl antibody, Autl4 antibody, apg4d antibody, autophagy related 4D cysteine peptidase antibody, autophagy related 4D, cysteine peptidase antibody, autophagy related 4D, cysteine peptidase L homeolog antibody, ATG4D antibody, Atg4d antibody, atg4d.L antibody

Background:

Synonyms: APG4D, AUTL4, APG4-D, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, ATG4D

Background: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Gene ID: 84971

UniProt: Q86TL0

Pathways: Autophagy