GAPDH antibody (AA 1-335)
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- Target See all GAPDH Antibodies
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Binding Specificity
- AA 1-335
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Reactivity
- Human, Mouse, Rat, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GAPDH antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Mouse, Pig, Rat
- Purification
- Purified by Protein A.
- Immunogen
- Recombinant full length human GAPDH
- Isotype
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α." in: Journal of cellular and molecular medicine, Vol. 22, Issue 9, pp. 4507-4521, (2019) (PubMed).
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: "Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer." in: Oncotarget, Vol. 8, Issue 24, pp. 39443-39459, (2018) (PubMed).
: "Expression and Clinical Significance of ILF2 in Gastric Cancer." in: Disease markers, Vol. 2017, pp. 4387081, (2018) (PubMed).
: "Irreversible electroporation-mediated shRNA knockdown of the HPV18 E6 gene suppresses cervical cancer growth in vitro and in vivo." in: Oncology letters, Vol. 14, Issue 2, pp. 1943-1949, (2017) (PubMed).
: "MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 1, pp. 124-136, (2017) (PubMed).
: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).
: "Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-β-mediated Nox4 expression." in: Redox biology, Vol. 11, pp. 390-402, (2017) (PubMed).
: "The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 38, Issue 3, pp. 1100-10, (2016) (PubMed).
: "Lrig1 is a positive prognostic marker in hepatocellular carcinoma." in: OncoTargets and therapy, Vol. 9, pp. 7071-7079, (2016) (PubMed).
: "Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5139458, (2016) (PubMed).
: "Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain-derived neurotrophic factor levels." in: Neural regeneration research, Vol. 11, Issue 10, pp. 1595-1602, (2016) (PubMed).
: "BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition." in: Oncology letters, Vol. 8, Issue 2, pp. 869-875, (2014) (PubMed).
: "BRAF-activated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma." in: Oncology letters, Vol. 8, Issue 5, pp. 1947-1952, (2014) (PubMed).
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Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α." in: Journal of cellular and molecular medicine, Vol. 22, Issue 9, pp. 4507-4521, (2019) (PubMed).
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Alternative Name
- GAPDH (GAPDH Products)
- Synonyms
- G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
- Background
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Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12 bs-0755R
Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
- Gene ID
- 2597
- UniProt
- P04406
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