PDK2 antibody

Catalog No. ABIN4912898

The Mouse Monoclonal anti-PDK2 antibody has been validated for WB and IHC (p). It is suitable to detect PDK2 in samples from Human and Mouse.

Quick Overview for PDK2 antibody (ABIN4912898)

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7E5

Product Details anti-PDK2 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This PDK2 monoclonal antibody is generated from mouse immunized with PDK2 recombinant protein.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Alternative Name: PDK2

Background:

Synonyms: PDHK2, PDKII, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDK2

Background: Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.

Gene ID: 5164

UniProt: Q15119

Pathways: PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect