Prosaposin antibody

Catalog No. ABIN4916372

Product Details anti-Prosaposin Antibody

Target: Prosaposin (PSAP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Reacts with human Saposin-B

Cross-Reactivity (Details): May cross react with proteins from other species due to sequence homology

Purification: Purified (protein A)

Immunogen: Synthetic peptide derived from an internal domain of human PSAP

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC1:200 - 1:1 000 WB1:500 - 1:5 000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water to reach 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: Proactivator Polypeptide Domain Saposin-B (PSAP) (PSAP Products)

Synonyms: GLBA antibody, SAP1 antibody, AI037048 antibody, SGP-1 antibody, SGP1A antibody, cb759 antibody, wu:fa14a06 antibody, wu:fb36e02 antibody, wu:fb58g07 antibody, PSAP antibody, PSP-A antibody, SFTPA1B antibody, SP-A antibody, prosaposin antibody, surfactant protein A1 antibody, PSAP antibody, Psap antibody, psap antibody, LOC100011417 antibody, LOC100304726 antibody, LOC100526719 antibody, SFTPA1 antibody

Background: The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins). Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase and galactosylceramide by beta-galactosylceramidase. Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A, GM1 gangliosides by beta-galactosidase and globotriaosylceramide by alpha-galactosidase A. Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator.

Gene ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity