Nucleolin antibody (pThr76)
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- Target See all Nucleolin (NCL) Antibodies
- Nucleolin (NCL)
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Binding Specificity
- pThr76
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nucleolin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- Specificity
- Reacts with Nucleolin with phoshothreonine 76 (pT76) from human and mouse
- Cross-Reactivity (Details)
- Do not cross react with phoshothreonine 84, 92 & 106 (pT76 and pT92). The detection of this P-NCL can be done during mitosis or in the centrosome during G1 phase,no signal in G2 phase.
- Purification
- Purified (protein A)
- Immunogen
- Synthetic peptide of human Nucleolin with a phoshothreonine at position 76
- Top Product
- Discover our top product NCL Primary Antibody
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- Application Notes
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Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
WB1:100 - 1:1000 IF1:50 - 1:300 - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Must be reconstituted in distilled water.
- Concentration
- 1 mg/mL
- Buffer
- Tris 0,1M, glycine 0,1M
- Storage
- 4 °C/-20 °C
- Storage Comment
- Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
- Expiry Date
- 6 months
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- Target
- Nucleolin (NCL)
- Alternative Name
- Nucleolin (NCL Products)
- Synonyms
- C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
- Background
- Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
- Gene ID
- 4691
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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