GSK3 alpha antibody (pTyr215, pTyr278)

Catalog No. ABIN492620

The Mouse Monoclonal anti-GSK3 alpha antibody is suitable to detect GSK3 alpha in samples from Human and Mouse. It has been validated for WB.

Quick Overview for GSK3 alpha antibody (pTyr215, pTyr278) (ABIN492620)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6D3

Product Details anti-GSK3 alpha Antibody

Binding Specificity: pTyr215, pTyr278

Specificity: This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.

Characteristics: Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278AA Sequence: N V S pY I C S

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocolsfor more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cellsDescription: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma(Human)Format: Lyophilized cell lysate from from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS with 0.09 % Sodium Azide/PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha

Background: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gene ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect