CLIP antibody (AA 25-39)

Catalog No. ABIN4949426

Product Details anti-CLIP Antibody

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Binding Specificity: AA 25-39

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLIP antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Purification: Protein G affinity chromatography

Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.

Clone: CLIP-1449

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.\. ELISA: order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE):0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CLIP

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Alternative Name: ACTH / CLIP (CLIP Products)

Background: ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Gene ID: 5443