MAG antibody

Catalog No. ABIN4951814

Product Details anti-MAG Antibody

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids EPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK of human MAG/SIGLEC4A were used as the immunogen for the Myelin-associated glycoprotein antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Myelin-associated glycoprotein antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Myelin-associated glycoprotein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG / Myelin-associated glycoprotein (MAG Products)

Synonyms: GMA antibody, S-MAG antibody, SIGLEC-4A antibody, SIGLEC4A antibody, siglec-4 antibody, MAG antibody, Gma antibody, siglec-4a antibody, myelin associated glycoprotein antibody, myelin-associated glycoprotein antibody, MAG antibody, mag antibody, Mag antibody

Background: MAG (Myelin-associated glycoprotein), also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is thought to be involved in the process of myelination. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway