Recombinant AKT1 antibody (C-Term)

Catalog No. ABIN4953292

The Rabbit Monoclonal anti-AKT1 antibody is suitable to detect AKT1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant AKT1 antibody (C-Term) (ABIN4953292)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RM252

Product Details anti-AKT1 Antibody

Binding Specificity: C-Term

Purpose: Recombinant AKT1 Antibody

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine

Characteristics: This recombinant AKT1 antibody may also react to bovine, mouse or rat AKT1, as predicted by immunogen homology

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the C-terminus of human Akt1 was used as the immunogen for this recombinant AKT1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant AKT1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background: This recombinant AKT1 antibody may also react to bovine, mouse or rat AKT1, as predicted by immunogen homology

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect