GTPase NRas antibody (AA 147-179)

Catalog No. ABIN3031131

This anti-GTPase NRas antibody is a Rabbit Polyclonal antibody detecting GTPase NRas in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for GTPase NRas antibody (AA 147-179) (ABIN3031131)

Target: GTPase NRas (NRAS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GTPase NRas Antibody

Binding Specificity: AA 147-179

Cross-Reactivity (Details): Expected species reactivity: Pig

Purification: Purified

Immunogen: A portion of amino acids 147-179 from the human protein was used as the immunogen for this NRAS antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NRAS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling