Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
Purification
Antigen affinity purified
Immunogen
This p-STAT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS727 of human STAT3.
KO Validated
STAT3
Reactivity: Human
WB, IHC, IP, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Titration of the p-STAT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the p-STAT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
STAT3
(Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Background
STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.