Synaptophysin antibody

Catalog No. ABIN498097

Product Details anti-Synaptophysin Antibody

Target: Synaptophysin (SYP)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody antibody detects endogenous levels of Synaptophysin protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blots: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: Synaptophysin / SYP (SYP Products)

Synonyms: syp-B antibody, xsypii antibody, MGC86267 antibody, MRXSYP antibody, A230093K24Rik antibody, AI848995 antibody, Syn antibody, p38 antibody, Syp1 antibody, mrxsyp antibody, syp antibody, xsypi antibody, synaptophysin S homeolog antibody, synaptophysin antibody, synaptophysin L homeolog antibody, synaptophysin a antibody, syp.S antibody, SYP antibody, syp antibody, Syp antibody, syp.L antibody, sypa antibody

Background: Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis.Synonyms: Major synaptic vesicle protein p38, Neuroendocrine Marker

Molecular Weight: approx. 38 kDa

Gene ID: 6855

NCBI Accession: NP_003170

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity