KCNJ3 antibody

Catalog No. ABIN498130

Product Details anti-KCNJ3 Antibody

Target: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNJ3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of KIR3.1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KCNJ3

Target: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Alternative Name: KCNJ3 (KCNJ3 Products)

Synonyms: GIRK1 antibody, KGA antibody, KIR3.1 antibody, Kcnf3 antibody, Kir3.1 antibody, girk1 antibody, potassium voltage-gated channel subfamily J member 3 antibody, potassium inwardly-rectifying channel, subfamily J, member 3 antibody, KCNJ3 antibody, Kcnj3 antibody

Background: G protein-coupled inwardly rectifying potassium channels (KIR3.1 through KIR3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, KIR3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbγ subunits from G protein heterotrimers bind to and regulate KIR3 channel activity. Gb3- and Gb4-containing Gbγ dimers bind directly to cytoplasmic domains of KIR3 proteins and increase the K+ current while Gb5-containing Gbγ dimers inhibit KIR3 K+ current. KIR3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates KIR3 tyrosine residues, effectively inactivating the KIR3 channels.Synonyms: G protein-activated inward rectifier potassium channel 1, GIRK1, Inward rectifier K(+) channel Kir3.1, Potassium channel, inwardly rectifying subfamily J member 3

Molecular Weight: approx. 43 kDa

Gene ID: 3760

NCBI Accession: NP_002230

UniProt: P48549