BUB1B antibody

Catalog No. ABIN498298

Product Details anti-BUB1B Antibody

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of ubR1 protein. (region surrounding Thr368)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B (BUB1B Products)

Synonyms: BUB1beta antibody, BUBR1 antibody, Bub1A antibody, MAD3L antibody, MVA1 antibody, SSK1 antibody, hBUBR1 antibody, AU045529 antibody, bub1b antibody, BUB1 antibody, BUB1-like antibody, BubR1 antibody, BubRI antibody, CG14030 antibody, CG14030/Bub1 antibody, Dmel\\CG14030 antibody, bub1 antibody, BUB1 mitotic checkpoint serine/threonine kinase B antibody, BUB1B, mitotic checkpoint serine/threonine kinase antibody, BUB1B, mitotic checkpoint serine/threonine kinase L homeolog antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody, Bub1 kinase antibody, BUB1B antibody, Bub1b antibody, bub1b.L antibody, bub1b antibody, Bub1 antibody

Background: The N-end rule relates the in vivo half-life of a protein to the composition of its N-terminal residues. The N-end rule pathway is part of the ubiquitin system, which involves a three-step mechanism. Proteins targeted for degradation are bound on their N-terminal residue by Ubr1 (also designated E3 α and N-recognin), which catalyzes the covalent attachment of ubiquitin to the protein substrate. Two zinc finger domains and the RING-H2 finger domain of Ubr1 are essential for substrate recognition. Ubr1 is located on mouse chromosome 2 and on human chromosome 15 in the syntenic region. Ubr1 is a 220 kDa protein that is ubiquitously expressed in adult mouse, with the highest expression detected in skeletal muscle and heart. In mouse embryo, Ubr1 is primarily expressed in the branchial arches and in the tail and limb buds.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1

Molecular Weight: approx. 130 kDa

Gene ID: 701

NCBI Accession: NP_001202

UniProt: O60566