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BUB3 antibody
BUB3
Reactivity: Human, Mouse, Rat
WB, IF, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-BUB3 Antibody
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Target
See all BUB3 Antibodies
BUB3
(Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
Reactivity
All reactivities for BUB3 antibodies
Human, Mouse, Rat
Host
All hosts for BUB3 antibodies
Rabbit
Clonality
All clonalities for BUB3 antibodies
Polyclonal
Conjugate
All conjugates for BUB3 antibodies
This BUB3 antibody is un-conjugated
Application
All applications for BUB3 antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody detects endogenous levels of Bub3 protein. (region surrounding His274)
Cross-Reactivity (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity chromatography
Purity
> 95 % by SDS-PAGE
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Discover our top product BUB3 Primary Antibody
Alternatives
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Application Details
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Application Notes
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for BUB3
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Target
BUB3
(Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
Alternative Name
BUB3 (BUB3 Products )
Synonyms
BUB3L antibody, hBUB3 antibody, Aa2-050 antibody, xbub3 antibody, AU019800 antibody, AU021329 antibody, AU043350 antibody, AW146323 antibody, C78067 antibody, BUB3, mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein L homeolog antibody, BUB3 antibody, Bub3 antibody, bub3.L antibody
Background
The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.Synonyms: Mitotic checkpoint protein BUB3
Molecular Weight
approx. 40 kDa
Gene ID
9184
NCBI Accession
NP_001007794
UniProt
O43684
Pathways
Maintenance of Protein Location
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