DOK1 antibody (pTyr362)

Catalog No. ABIN498758

Product Details anti-DOK1 Antibody

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Binding Specificity: pTyr362

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of DOK1 protein only when phosphorylated at Tyr362.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: DOK1 / p62 Dok (DOK1 Products)

Synonyms: P62DOK antibody, Dok-1 antibody, AW557123 antibody, p62DOK antibody, dok1 antibody, zgc:76914 antibody, docking protein 1 antibody, docking protein 1b antibody, DOK1 antibody, Dok1 antibody, dok1b antibody

Background: Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9,22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of Synonyms: Docking protein 1, Downstream of tyrosine kinase 1, p62(dok), pp62

Molecular Weight: approx. 62 kDa

Gene ID: 1796

NCBI Accession: NP_001372

UniProt: Q99704