STAT1 antibody (pSer727)

Catalog No. ABIN498804

The Rabbit Polyclonal anti-STAT1 antibody (ABIN498804) specifically detects STAT1 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for STAT1 antibody (pSer727) (ABIN498804)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STAT1 Antibody

Binding Specificity: pSer727

Specificity: This antibody detects endogenous levels of p-STAT1 protein only when phosphorylated at Ser727.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1

Background: TAT1 is known to associate with the transcription factors p48, Sp1, and p300. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. STAT1 alpha and STAT1 beta isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1 beta lacks 38 carboxy terminal amino acids. Contained within these 38 terminal amino acids of STAT1 alpha is a critical serine residue (Ser 727) whose phosphorylation is required for maximal transcription activity induced by IFN gamma.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

Molecular Weight: approx. 87 kDa

Gene ID: 6772

NCBI Accession: NP_009330

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events