PRKAA1 antibody

Catalog No. ABIN498910

Product Details anti-PRKAA1 Antibody

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of AMPK1/2 protein. (region surrounding Arg493)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: AMPK2

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 17200. Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1 (PRKAA1 Products)

Synonyms: AMPK antibody, AMPKa1 antibody, cb116 antibody, im:7154392 antibody, sb:cb130 antibody, wu:fa94c10 antibody, AMPK1 antibody, AMPKalpha1 antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, C130083N04Rik antibody, ampk antibody, ampka1 antibody, PRKAA1 antibody, protein kinase AMP-activated catalytic subunit alpha 1 antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antibody, PRKAA1 antibody, prkaa1 antibody, Prkaa1 antibody, prkaa1.L antibody

Background: AMPK is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATPconsuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein. The human AMPKgamma1 gene encodes a 331 amino acid protein. Human AMPKgamma2 and AMPKgamma3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK alpha-1 chain, AMPK1

Molecular Weight: approx. 63 kDa

Gene ID: 5562

NCBI Accession: NP_006242

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect