Protein A antibody (AbBy Fluor® 750)

Catalog No. ABIN5009282

Product Details anti-Protein A Antibody (AbBy Fluor® 750)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Bacteria

Cross-Reactivity (Details): S. aureus

Purification: Purified by Protein A.

Immunogen: Recombinant protein A

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein antibody, repB antibody

Background:

Synonyms: Immunoglobulin G binding protein A, Staphylococcal protein A, SPA, gG-binding protein A, Staphylococcus aureus.

Background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.