IKKi/IKKe antibody (AA 2-203)

Catalog No. ABIN5027294

Product Details anti-IKKi/IKKe Antibody

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Binding Specificity: AA 2-203

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKKi/IKKe antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G Chromatography

Immunogen: A partial length recombinant Ikke protein (amino acids 2-203) was used as the immunogen for this antibody.

Clone: ABM13C7

Isotype: IgG2a kappa

Application Details

Application Notes: WB: 2-4 μg/mL, IHC: 10-20 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for IKKi/IKKe

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Alternative Name: Ikke/Ikki/TBK1 (IKBKE Products)

Synonyms: zgc:100844 antibody, IKBKE antibody, MGC130907 antibody, IKK-E antibody, IKK-i antibody, IKKE antibody, IKKI antibody, AW558201 antibody, IKKepsilon antibody, Ikki antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antibody, inhibitor of kappaB kinase epsilon antibody, IKBKE antibody, ikbke antibody, ikbke.L antibody, Ikbke antibody

Background: Ikke is a member of the IKK (IKappaB kinase) family that has been identified as an oncogenic protein and found to be up-regulated in breast cancer, ovarian cancer and prostate cancer. The Ikke protein has 33 % and 31 % identity at the amino acid level with Ikka and Ikkb, respectively and is primarily activated by interferon and mediates interferon signaling. The activated Ikke stimulates the transcription factor interferon regulatory factors 3 and phosphorylates signal transducer and activator of transcription. Ikke also shares some function with Ikka and Ikkb to activate NF- KappaB pathway by phosphorylation and degradation of IKappaBalpha. However, Ikke mainly mediates NF-KappaB activation induced by interferon, phorbol 12-myristate 13-acetate or the T-cell receptor, but not by tumor necrosis factor and interleukin 1, which activate Ikka and Ikkb. Ikke is predominantly expressed and active in peripheral blood leukocytes, pancreas, thymus, and spleen and contributes to IRF as well as NF-KappaB activation.

Molecular Weight: 76

Gene ID: 9641

UniProt: Q14164

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades