Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purpose
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AURKB.
Specificity
Reactivity occurs against human Aurora Kinase B pT232 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A.
Cross-Reactivity
Chimpanzee, Cow, Dog, Human, Mouse, Pig, Rat
Characteristics
Antibody Reactive Against Synthetic Peptide.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T232 of human AURKB.
ELISA (1:10000-1:50000) Western Blot (1:250-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Yasui, Urano, Kawajiri, Nagata, Tatsuka, Saya, Furukawa, Takahashi, Izawa, Inagaki: "Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12997-3003, (2004) (PubMed).
Kimura, Uchida, Takano, Kitagawa, Okano: "Cell cycle-dependent regulation of the human aurora B promoter." in: Biochemical and biophysical research communications, Vol. 316, Issue 3, pp. 930-6, (2004) (PubMed).
Gruneberg, Neef, Honda, Nigg, Barr: "Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2." in: The Journal of cell biology, Vol. 166, Issue 2, pp. 167-72, (2004) (PubMed).