Cyclin B1 antibody (pSer126)

Catalog No. ABIN538183

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Binding Specificity: pSer126

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNB1.

Specificity: This antibody is specific to phosphorylated human Cyclin B1 protein at the pS126 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Cross-Reactivity: Chimpanzee, Dog, Human, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S126 of human CCNB1.

Application Details

Application Notes: ELISA (1:50000)
Western Blot (1:100-1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Cyclin B1 antibody (ABIN538183)

Innocente, Lee: "p53 is a NF-Y- and p21-independent, Sp1-dependent repressor of cyclin B1 transcription." in: FEBS letters, Vol. 579, Issue 5, pp. 1001-7, (2005) (PubMed).

Davy, Jackson, Raj, Peh, Southern, Das, Sorathia, Laskey, Middleton, Nakahara, Wang, Masterson, Lambert, Cuthill, Millar, Doorbar: "Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes." in: Journal of virology, Vol. 79, Issue 7, pp. 3998-4011, (2005) (PubMed).

Torgler, Jakob, Ontsouka, Nachbur, Mueller, Green, Brunner: "Regulation of activation-induced Fas (CD95/Apo-1) ligand expression in T cells by the cyclin B1/Cdk1 complex." in: The Journal of biological chemistry, Vol. 279, Issue 36, pp. 37334-42, (2004) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1 (CCNB1 Products)

Synonyms: ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody

Gene ID: 891

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase