GLI2 antibody (Internal Region)

Catalog No. ABIN538409

Product Details anti-GLI2 Antibody

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of Gli2.

Specificity: This affinity purified antibody is directed against mouse Gli-2 protein.

Cross-Reactivity: Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: A synthetic peptide corresponding to internal region of mouse Gli2.

Isotype: IgG

Application Details

Application Notes: ELISA (1:15000-1:60000)
Western Blot (1:500-1:2000)
Immunohistochemistry (2-20 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-GLI2 antibody (ABIN538409)

Kim, Huang, Mo: "Gli3 null mice display glandular overgrowth of the developing stomach." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 234, Issue 4, pp. 984-91, (2005) (PubMed).

Dillon, Fujita, Matise, Jarjour, Kennedy, Kollmus, Arnold, Weiner, Sanes, Kaprielian: "Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 44, pp. 10119-30, (2005) (PubMed).

McDermott, Gustafsson, Elsam, Hui, Emerson, Borycki: "Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation." in: Development (Cambridge, England), Vol. 132, Issue 2, pp. 345-57, (2004) (PubMed).

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2 (GLI2 Products)

Synonyms: HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody

Gene ID: 14633

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis