POLK antibody (AA 817-830)

Catalog No. ABIN539465

Product Details anti-POLK Antibody

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Binding Specificity: AA 817-830

Reactivity: Human, Mouse, Chimpanzee, Orang-Utan

Host: Goat

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Goat polyclonal antibody raised against synthetic peptide of POLK.

Specificity: Expect reactivity with multiple splice variants of this protein.

Cross-Reactivity: Chimpanzee, Human, Mouse, Orang-Utan

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: A synthetic peptide corresponding to amino acids 817-830 of human POLK.

Application Details

Application Notes: ELISA (1:2000-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-POLK antibody (ABIN539465)

Uljon, Johnson, Edwards, Prakash, Prakash, Aggarwal: "Crystal structure of the catalytic core of human DNA polymerase kappa." in: Structure (London, England : 1993), Vol. 12, Issue 8, pp. 1395-404, (2004) (PubMed).

Yasui, Suzuki, Miller, Matsuda, Matsui, Shibutani: "Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases." in: Journal of molecular biology, Vol. 344, Issue 3, pp. 665-74, (2004) (PubMed).

Haracska, Prakash, Prakash: "A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair." in: Genes & development, Vol. 17, Issue 22, pp. 2777-85, (2003) (PubMed).

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: DNA Polymerase kappa (POLK Products)

Synonyms: DINB1 antibody, DINP antibody, POLQ antibody, Dinb1 antibody, POLK antibody, si:ch211-254o18.3 antibody, DNA polymerase kappa antibody, polymerase (DNA directed) kappa antibody, polymerase (DNA directed), kappa antibody, polymerase (DNA directed) kappa L homeolog antibody, POLK antibody, Polk antibody, polk antibody, Tb11.01.0080 antibody, Tb11.01.0010 antibody, Tb11.01.0020 antibody, Tb11.01.0030 antibody, Tb11.01.0040 antibody, Tb11.01.0050 antibody, Tb11.12.0001 antibody, Tb11.12.0002 antibody, Tb11.12.0003 antibody, polk.L antibody, polk-1 antibody

Gene ID: 51426

Pathways: DNA Damage Repair