SMAD3 antibody (AA 417-425)

Catalog No. ABIN539484

Product Details anti-SMAD3 Antibody

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Binding Specificity: AA 417-425

Reactivity: Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.

Specificity: ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members.

Cross-Reactivity: Chicken, Cow, Frog, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: A synthetic peptide corresponding to amino acids 417-425 of human SMAD3.

Application Details

Application Notes: ELISA (1:10000-1:40000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-SMAD3 antibody (ABIN539484)

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).

Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." in: Nature, Vol. 425, Issue 6958, pp. 577-84, (2003) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3 (SMAD3 Products)

Synonyms: HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

Gene ID: 4088

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy