ERBB3 antibody (pTyr1328)

Catalog No. ABIN540462

Product Details anti-ERBB3 Antibody

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Binding Specificity: pTyr1328

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB3.

Sequence: PDYpWH

Specificity: Her3/ErbB3 (phospho-Tyr1328) Antibody detects endogenous levels of Her3/ErbB3 only when phosphorylated at tyrosine 1328.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1328 of human ERBB3.

Application Details

Application Notes: Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS (without Mg²⁺ and Ca²⁺), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-ERBB3 antibody (ABIN540462)

Kobayashi, Iwamatsu, Shinohara-Kanda, Ihara, Fukui: "Activation of ErbB3-PI3-kinase pathway is correlated with malignant phenotypes of adenocarcinomas." in: Oncogene, Vol. 22, Issue 9, pp. 1294-301, (2003) (PubMed).

Holbro, Beerli, Maurer, Koziczak, Barbas, Hynes: "The ErbB2/ErbB3 heterodimer functions as an oncogenic unit: ErbB2 requires ErbB3 to drive breast tumor cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 15, pp. 8933-8, (2003) (PubMed).

Yarden, Sliwkowski: "Untangling the ErbB signalling network." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 2, pp. 127-37, (2001) (PubMed).

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: ERBB3 / HER3 (ERBB3 Products)

Synonyms: ErbB-3 antibody, HER3 antibody, LCCS2 antibody, MDA-BF-1 antibody, c-erbB-3 antibody, c-erbB3 antibody, erbB3-S antibody, p180-ErbB3 antibody, p45-sErbB3 antibody, p85-sErbB3 antibody, ERBB3 antibody, C76256 antibody, Erbb-3 antibody, Erbb3r antibody, Her3 antibody, nuc-ErbB3 antibody, erb-b2 receptor tyrosine kinase 3 antibody, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody, ERBB3 antibody, Erbb3 antibody

Gene ID: 2065

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway