SKP1
Reactivity: Human
WB, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Wu, Xu, Schulman, Jeffrey, Harper, Pavletich: "Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase." in: Molecular cell, Vol. 11, Issue 6, pp. 1445-56, (2003) (PubMed).
Piva, Liu, Chiarle, Podda, Pagano, Inghirami: "In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8375-87, (2002) (PubMed).
Matsuzawa, Reed: "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses." in: Molecular cell, Vol. 7, Issue 5, pp. 915-26, (2001) (PubMed).