PKC epsilon antibody (AA 213-243)

Catalog No. ABIN545461

Product Details anti-PKC epsilon Antibody

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Binding Specificity: AA 213-243

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of PRKCE.

Cross-Reactivity: Human

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to amino acids 213-243 at N-terminus of human PRKCE.

Application Details

Application Notes: ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-PKC epsilon antibody (ABIN545461)

Tang, Bouyer, Mykoniatis, Buschmann, Matlin, Matthews: "Activated PKC{delta} and PKC{epsilon} inhibit epithelial chloride secretion response to cAMP via inducing internalization of the Na+-K+-2Cl- cotransporter NKCC1." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 34072-85, (2010) (PubMed).

Gray, Zhou, Schafhalter-Zoppoth, Zhu, Mochly-Rosen, Messing: "Preservation of base-line hemodynamic function and loss of inducible cardioprotection in adult mice lacking protein kinase C epsilon." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3596-604, (2004) (PubMed).

Ling, Trollér, Zeidman, Lundberg, Larsson: "Induction of neurites by the regulatory domains of PKCdelta and epsilon is counteracted by PKC catalytic activity and by the RhoA pathway." in: Experimental cell research, Vol. 292, Issue 1, pp. 135-50, (2004) (PubMed).

McJilton, Van Sikes, Wescott, Wu, Foreman, Gregory, Weidner, Harris Ford, Morgan Lasater, Mohler, Terrian: "Protein kinase Cepsilon interacts with Bax and promotes survival of human prostate cancer cells." in: Oncogene, Vol. 22, Issue 39, pp. 7958-68, (2003) (PubMed).

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PRKCE (PRKCE Products)

Synonyms: PKCE antibody, nPKC-epsilon antibody, 5830406C15Rik antibody, PKC[e] antibody, PKCepsilon antibody, Pkce antibody, R75156 antibody, fj59b07 antibody, prkce antibody, wu:fj59b07 antibody, pkce antibody, pkc-epsilon antibody, PRKCH antibody, protein kinase C epsilon antibody, protein kinase C, epsilon antibody, protein kinase C, epsilon a antibody, PRKCE antibody, Prkce antibody, prkcea antibody, prkce antibody

Gene ID: 5581

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling