Cyclin E1 antibody (C-Term)

Catalog No. ABIN545776

Product Details anti-Cyclin E1 Antibody

Target: Cyclin E1 (CCNE1)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of CCNE1.

Cross-Reactivity: Human

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CCNE1.

Application Details

Application Notes: ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Cyclin E1 antibody (ABIN545776)

Honda, Lowe, Dubinina, Skamnaki, Cook, Brown, Johnson: "The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles." in: The EMBO journal, Vol. 24, Issue 3, pp. 452-63, (2005) (PubMed).

Wingate, Zhang, McGarhen, Bedrosian, Harper, Keyomarsi: "The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15148-57, (2005) (PubMed).

Ausserlechner, Obexer, Geley, Kofler: "G1 arrest by p16INK4A uncouples growth from cell cycle progression in leukemia cells with deregulated cyclin E and c-Myc expression." in: Leukemia, Vol. 19, Issue 6, pp. 1051-7, (2005) (PubMed).

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1 (CCNE1 Products)

Synonyms: CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody

Gene ID: 898

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases