DOK1 antibody (pSer450)

Catalog No. ABIN548455

Product Details anti-DOK1 Antibody

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Binding Specificity: pSer450

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of DOK1.

Specificity: This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S450 of human DOK1.

Application Details

Application Notes: ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-DOK1 antibody (ABIN548455)

Woodring, Meisenhelder, Johnson, Zhou, Field, Shah, Bladt, Pawson, Niki, Pandolfi, Wang, Hunter: "c-Abl phosphorylates Dok1 to promote filopodia during cell spreading." in: The Journal of cell biology, Vol. 165, Issue 4, pp. 493-503, (2004) (PubMed).

Lee, Andrieu, Saltel, Destaing, Auclair, Pouchkine, Michelon, Salaun, Kobayashi, Jurdic, Kieff, Sylla: "IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 50, pp. 17416-21, (2004) (PubMed).

Kashige, Carpino, Kobayashi: "Tyrosine phosphorylation of p62dok by p210bcr-abl inhibits RasGAP activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2093-8, (2000) (PubMed).

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: DOK1 / p62 dok (DOK1 Products)

Synonyms: P62DOK antibody, Dok-1 antibody, AW557123 antibody, p62DOK antibody, dok1 antibody, zgc:76914 antibody, docking protein 1 antibody, docking protein 1b antibody, DOK1 antibody, Dok1 antibody, dok1b antibody

Gene ID: 1796