Connexin 43/GJA1 antibody (pSer368)

Catalog No. ABIN550176

Product Details anti-Connexin 43/GJA1 Antibody

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Binding Specificity: pSer368

Reactivity: Human, Rat, Mouse, Dog, Cow, Chicken, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gja1.

Cross-Reactivity: Chicken, Cow, Dog, Guinea Pig, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S368 of rat Gja1.

Application Details

Application Notes: Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50 % glycerol, 10 % BSA)

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Connexin 43/GJA1 antibody (ABIN550176)

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).

Bao, Altenberg, Reuss: "Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation." in: American journal of physiology. Cell physiology, Vol. 286, Issue 3, pp. C647-54, (2004) (PubMed).

Bao, Reuss, Altenberg: "Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368." in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 20058-66, (2004) (PubMed).

Yogo, Ogawa, Akiyama, Ishida, Takeya: "Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells." in: FEBS letters, Vol. 531, Issue 2, pp. 132-6, (2002) (PubMed).

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: GJA1 / Cx43 (GJA1 Products)

Synonyms: AVSD3 antibody, CX43 antibody, DFNB38 antibody, GJAL antibody, HLHS1 antibody, HSS antibody, ODDD antibody, Cx43 antibody, AU042049 antibody, AW546267 antibody, Cnx43 antibody, Cx43alpha1 antibody, Gja-1 antibody, Npm1 antibody, connexin43 antibody, cx43a1 antibody, gja1 antibody, zfCx43.3 antibody, connexin 43 antibody, gja1-A antibody, cx43 antibody, gjal antibody, oddd antibody, dfnb38 antibody, gap junction protein alpha 1 antibody, gap junction protein, alpha 1 antibody, connexin 43 antibody, gap junction protein alpha 1 L homeolog antibody, CONNEXIN 43 protein antibody, GJA1 antibody, Gja1 antibody, cx43 antibody, gja1.L antibody, gja1 antibody, CONNEXIN 43 antibody

Gene ID: 24392

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization