BCKDK antibody (AA 120-151)

Catalog No. ABIN5530501

Product Details anti-BCKDK Antibody

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Binding Specificity: AA 120-151

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This BCKDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-151 amino acids from the Central region of human BCKDK.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BCKDK

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK (BCKDK Products)

Synonyms: cb233 antibody, zgc:55984 antibody, wu:fa04g07 antibody, wu:fb80b08 antibody, MGC78818 antibody, BCKDK antibody, AI327402 antibody, BCKDKD antibody, BDK antibody, branched chain ketoacid dehydrogenase kinase antibody, branched chain ketoacid dehydrogenase kinase S homeolog antibody, bckdk antibody, bckdk.S antibody, BCKDK antibody, Bckdk antibody

Background: The second major step in the catabolism of the branched-chain amino acids, isoleucine, leucine, and valine, is irreversibly catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD), an inner-mitochondrial enzyme complex composed of 3 catalytic components: a branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). The complex also contains 2 enzymes that regulated the state of activity of the BCKD complex: a kinase (BCKDK), and a phosphorylase. The ubiquitiously expressed kinase contains 1 histidine kinase domain. Maple syrup urine disease (MSUD) is a pathology secondary to an enzyme defect in the catabolic pathway of leucine, isoleucine, and valine. Accumulation of these amino acids and their corresponding keto acids results in encephalopathy and progressive neurodegeneration in infants not treated for MSUD.

Molecular Weight: 46 kDa

Gene ID: 10295

UniProt: O14874

Pathways: SARS-CoV-2 Protein Interactome