IL-10 antibody (AA 27-53)

Catalog No. ABIN5531466

Product Details anti-IL-10 Antibody

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Binding Specificity: AA 27-53

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10 (IL10 Products)

Synonyms: CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody

Background: The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Molecular Weight: 21 kDa

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints