ATP6V0B antibody (AA 104-131)

Catalog No. ABIN5535828

Product Details anti-ATP6V0B Antibody

Target: ATP6V0B (ATPase, H+ Transporting, Lysosomal 21kDa, V0 Subunit B (ATP6V0B))

Binding Specificity: AA 104-131

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATP6V0B

Target: ATP6V0B (ATPase, H+ Transporting, Lysosomal 21kDa, V0 Subunit B (ATP6V0B))

Alternative Name: ATP6V0B (ATP6V0B Products)

Synonyms: ATP6F antibody, HATPL antibody, VMA16 antibody, 2310024H13Rik antibody, Atp6f antibody, ATPase H+ transporting V0 subunit b antibody, ATPase, H+ transporting, lysosomal V0 subunit B antibody, ATPase H+ transporting V0 subunit B antibody, ATP6V0B antibody, Atp6v0b antibody

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.

Molecular Weight: 21 kDa

Gene ID: 533

UniProt: Q99437

Pathways: Transition Metal Ion Homeostasis, Proton Transport