Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human PIGA.
PIGA
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
PIGA
(Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))
GPI3 antibody, MCAHS2 antibody, PIG-A antibody, AI194334 antibody, Pig-a antibody, im:6911675 antibody, zgc:56589 antibody, phosphatidylinositol glycan anchor biosynthesis class A antibody, phosphatidylinositol glycan anchor biosynthesis, class A antibody, PIGA antibody, Piga antibody, piga antibody
Background
This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.