CD47 antibody (Internal Region)

Catalog No. ABIN5539493

This Goat Polyclonal antibody specifically detects CD47 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD47 antibody (Internal Region) (ABIN5539493)

Target: CD47

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CD47 Antibody

Binding Specificity: Internal Region

Purpose: CD47

Sequence: SNQKTIQPPR K

Specificity: This antibody is expected to recognize both reported isoforms (NP_001768.1, NP_942088.1).

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Hippocampus lysates and 60 kDa in lysates of cell lines HeLa and Jurkat (calculated MW of 35.2 kDa according to NP_001768.1). The observed molecular weights correspond to glycosylated forms. Recommended con

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: CD47, CD47 Molecule, IAP, MER6, OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic surface determinant protein OA3, integrin associ

Gene ID: 961

NCBI Accession: NP_001768, NP_942088