MAPK6 antibody (C-Term, Internal Region)

Catalog No. ABIN5541249

Product Details anti-MAPK6 Antibody

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Binding Specificity: C-Term, Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: C-HSPVGSPLKS IQ

Specificity: Recognizes MAPK6 / ERK3.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.

Application Details

Application Notes: Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N. Western blots of the IP were labelled with Rabbit anti-ERK3 serum (CST) or with Mouse anti-MK5 (SC). An approx 100 kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Tris saline, pH ~7.3 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: mapk6,erk3 (MAPK6 Products)

Synonyms: ERK3 antibody, HsT17250 antibody, PRKM6 antibody, p97MAPK antibody, 2610021I23Rik antibody, D130053K17Rik antibody, Erk3 antibody, Mapk4 antibody, Mapk63 antibody, Prkm4 antibody, Prkm6 antibody, ATMAPK6 antibody, ATMPK6 antibody, F18O19.10 antibody, MAP KINASE 6 antibody, MAP kinase 6 antibody, MAPK6 antibody, SUSERK3 antibody, erk3 antibody, p97mapk antibody, si:ch211-235f12.1 antibody, mitogen-activated protein kinase 6 antibody, MAP kinase 6 antibody, MAPK6 antibody, Mapk6 antibody, MPK6 antibody, mapk6 antibody

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C