GFP antibody

Catalog No. ABIN5541513

The Mouse Monoclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for WB, IF, IP, ICC and IHC (p).

Quick Overview for GFP antibody (ABIN5541513)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1E4

Product Details anti-GFP Antibody

Specificity: This antibody detects GFP. It reacts with EBFP, SEBFP, ECFP, SECFP, EGFP, SEGFP cpSEGFP, EYFP, Venus, cpVenus, R-pericam, and Sapphire.

Purification: Protein A Agarose

Immunogen: Recombinant full length GFP protein (246 aa)

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 5 μg/Sample. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Immunocytochemistry: 2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: gfp

Background: Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research.

UniProt: P42212