MSLN
Reactivity: Human
IHC (f)
Host: Mouse
Monoclonal
Biotin
Application Notes
ELISA: 1/10,000-1/50,000. Immunoprecipitaion. Western blot: 1/1,000. Expect a band approximately 40 kDa in size corresponding to mature mesothelin in theappropriate cell lysate or extract. Immunohistochemistry on Paraffin Sections: 1/100. Archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrievalis recommended. Block tissues with 1 % BSA in PBS for 30 min at 23 °C. Antibodies are diluted in 1 % BSA andreacted with tissue for 60 min at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Mesothelin is a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidylinositol-anchored protein that composes the mature portion and an NH2-terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.Synonyms: CAK1 antigen, MPF, MSLN, Pre-pro-megakaryocyte-potentiating factor