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Rhodopsin antibody (N-Term)

RHO WB, IP, IF, IHC (fro), EIA Host: Mouse Monoclonal 4D2 unconjugated
Catalog No. ABIN487510
  • Target See all Rhodopsin (RHO) Antibodies
    Rhodopsin (RHO)
    Binding Specificity
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    Please inquire
    Host
    • 39
    • 26
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 35
    • 33
    • 1
    Monoclonal
    Conjugate
    • 28
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Rhodopsin antibody is un-conjugated
    Application
    • 52
    • 42
    • 35
    • 25
    • 14
    • 12
    • 10
    • 8
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    N-terminus of Rhodopsin.
    Cross-Reactivity (Details)
    Species reactivity (tested):All Mammalian species (Human, Mouse, Rat, Bovine, Pig, etc), Most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
    Characteristics
    Synonyms: RHO, Opsin-2, OPN2
    Purification
    Protein G Chromatography.
    Immunogen
    Bovine Rhodopsin.
    Clone
    4D2
    Isotype
    IgG1
    Top Product
    Discover our top product RHO Primary Antibody
  • Application Notes
    ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Rhodopsin (RHO)
    Alternative Name
    Rhodopsin (RHO Products)
    Synonyms
    CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody
    Target Type
    Chemical
    Background
    Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO
    Gene ID
    6010
    UniProt
    P08100
    Pathways
    WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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