DNMT3L antibody (N-Term)

Catalog No. ABIN492471

Product Details anti-DNMT3L Antibody

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Binding Specificity: AA 1-387, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Detects ~45 kDa protein. No cross-reactivity against DNMT3a or DNMT3B.

Characteristics: Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1-387 of Human DNMT3L (accession number BAA95556).

Clone: S117-9

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of DNMT3L in 10 μg of COS cell transiently transfectedwith Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using Goatanti-Mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L (TRDMT1 Products)

Synonyms: DNMT3L antibody, D6Ertd14e antibody, ecat7 antibody, DNA methyltransferase 3 like antibody, DNA (cytosine-5-)-methyltransferase 3-like antibody, DNMT3L antibody, Dnmt3l antibody

Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Gene ID: 29947

UniProt: Q9UJW3